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KMID : 0351619720130020185
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Kyungpook Medical Journal
1972 Volume.13 No. 2 p.185 ~ p.195
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An Electron Microscopic Study of Early Changes of Cytoplasmic Organells Involved to Protein Synthesis by DL-Ethionine in Rat Liver
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Abstract
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It is well known that ethionine treatment induces decrease of ATP concentration and resultant inhibition of RNA and protein s}~nthesis in the liver cell. Ethionine traps adeno:~ine forming S-adenosylethionine which results in deficiency of available adenosine for synthesis of ATP. But the relation of the morphological changes to the biochemical changes is still uncertain. In an atte_np to clarify the initial ch-anges of the cytoplasmic organelle involved in protein synthesis, the author injected intraperitoneally 4 cc of 1.25% ethionine to the rats of fasted and nonfasted. Animals were killed at 10, 30, 6~, 120 and 240 minutes after the i:´ jection and the livers were examined by light and electron microspcopes.
The liver cells showed shedding of membrane-bound ribosomes, decrease of polysomes with loss of their filaments, and increase of monoribosome tivithin 10 minutes after intraperitoneal injection of ethionine. Also noted were dilatation of rough surfaced endoplasmic reticulum and atrophy of Golgi complex. The changE~s were more marked in later time. Such changes of cytoplasmic organells, which could be recogn-ized within such early time, suggested the possibility of direct action of ethionine to the cytoplasmic organells, besides the toxic effects through ATP depletion or other metabobic derangements.
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